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Agenda
Day One - 16 October
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08:55 |
Welcome |
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Technology (Session 1)
Chaired by Jörg Baumbach, ISAS Institute for Analytical Sciences |
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09:00 |
Optimizing Metabolic Profiling Approaches for Systems Biology Applications
Elaine Holmes, Professor of Chemical Biology, Imperial College London
Strategies for optimization of mathematical modelling of high-resolution spectroscopic data are presented with a view to defining metabolic phenotypes associated with health and disease. Such approaches can dramatically enhance extraction of latent biological information and provide insight at the systems level. |
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09:30 |
Presentation to be confirmed |
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10:00 |
Coffee Break and Networking in the Exhibition Hall |
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Environmental and Plant Metabolomics
Chaired by Jörg Baumbach, ISAS Institute for Analytical Sciences |
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10:45 |
Metabolomics of Resistance in Plants Against Biotic Stress
Ajjamada Kushalappa, Associate Professor, McGill University
Plants produce both constitutive and induced metabolites to defend against pathogens. The metabolic profiling, using gas or liquid chromatography and high resolution mass spectrometry, can enable identification of the superior repertoire of metabolic activity in resistant than in susceptible plant genotypes. These metabolites can be used as biomarkers to screen breeding lines for stress resistance. |
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11:15 |
Development of Biomarker Protocols for the Assessment of Environmental Air Quality Changes using the Reindeer Lichen Cladonia portentosa
Sabine Freitag, PhD Researcher, Environmental Research Institute (ERI) Thurso (Scotland)/North Highland College/UHI
The present study has been looking at the affects of wet nitrogen deposition on the metabolic response of the reindeer lichen Cladonia portentosa applying high-throughput metabolomic methods such as FT-IR and LC-ESI-TOF and multivariate modelling approaches. |
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11:45 |
A Metabolomic Approach for Analysing Plant-Herbivore Interactions
Emma Marsden-Edwards, Metabolic Profiling Market Development Manager, Waters Corporation |
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12:15 |
Lunch and Networking in the Exhibition Hall |
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13:15 |
Poster Viewing |
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Technology (Session 2)
Chaired by Elaine Holmes, Imperial College London |
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14:00 |
Cellular Metabolomic Chemical Imaging for Fingerprinting and Targeted Analysis: The Application of Focal Plane Array Fourier-Transform Infrared Microscopy to Study Plant Development
Simon Thain, Fellow, University of the Highlands and Islands (UHI)
The paper presents the use of Infrared microscopy (FT-IR) for "chemical imaging" of the metabolome at cellular resolution. An example of its application for plant embryo development is presented. |
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14:30 |
Data Processing Required to Conduct Salivary Metabonomics
Michael Cannon, Scientist, Procter & Gamble
The work presented will show how it has been possible to carry out full metabonomics on saliva to obtain reproducible results which give insights into how oral care products affect the salivary compositions of consumers. |
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15:00 |
NMR – Capillary Electrophoresis: Yin and Yang of Metabolic Profiling
Oleg Mayboroda, Assistant Professor, Leiden University Medical Centre |
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15:30 |
Coffee Break and Networking in the Exhibition Hall |
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16:15 |
A Lipidomic Study of the PPAR-a Null Mouse using Ion-Mobility Mass Spectrometry
John Shockcor, Visiting Fellow, University of Cambridge |
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16:45 |
Effect of Dietary Sources and Dietary Components on the Metabolite Profile of Biofluids
Hanne Christine Bertram, Senior Scientist, University of Aarhus
Proton NMR-based applications for examining the metabolic effects of dietary compounds are presented. Emphasis will be on intervention studies where metabolic profiling of biofluids by 1H NMR spectroscopy is applied to elucidate the biochemical effects and identify potential dietary biomarkers. |
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17:15 |
Drinks Reception in the Exhibition Hall |
Day Two - 17 October
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Disease
Chaired by Julian Griffin, University of Cambridge |
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09:00 |
Monitoring Dyslipidaemia in Mouse Models of Atherosclerosis by Metabolomics
Julian Griffin, Research Group Leader, University of Cambridge
Cardiovascular disease is the leading cause of mortality in modern society. The group has used metabolomics to profile mouse models representing atherosclerosis including the ApoE null mouse, LDL-receptor null mouse and an ATM/ApoE null mouse to investigate disease development. |
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09:30 |
Metabolomics of Synchronized Colon Cancer Cell Lines
Sasidhar Maddula, PhD Student, ISAS Institute for Analytical Sciences |
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10:00 |
Coffee Break and Networking in the Exhibition Hall |
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10:45 |
Metabolic Profiling for AK-1 (which Protects Cells against Oxidative Stress) and for AK-2 (which Protects Cells against a Broad-Spectrum of Viral Infection)
Grace Wong, President, Chief Scientific Officer, ActoKine Therapeutics
ActoKine has identified ActoKine-2 (AK-2) which protects cells against infection by a broad spectrum of viruses. In collaborative work with NIAID and other laboratories to test the efficacy of AK-2 against a variety of viruses, AK-2 was found to have a protective potency greater than interferons against RNA, DNA & retrovirus in vitro & in vivo. |
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11:15 |
Metabolic Profiling in Breath and Lung Cancer Research
Jörg Baumbach, Director, Department of Metabolomics, ISAS Institute for Analytical Sciences
Breathograms from GC/MS trained ion mobility spectra (MCC/IMS) will show the possibility to investigate human breath samples of 10 mL directly with respect to various lung diseases including lung cancer at different stages by volatile metabolites. |
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11:45 |
Metabonomic Analysis of Dexamethasone-Induced Cleft Palate in Pregnant C57BL/6J Mice
Jinglin Zhou, Post-Graduate Student, Sichuan University
A metabonomics approach was used to discriminate between dexamethasone-induced cleft palate in pregnant C57BL/6J mice and controls, at the same time, the relationship between the change of endogenous small molecular metabolites in maternal plasma and the incidence of cleft palate were studied. |
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12:15 |
Lunch and Networking in the Exhibition Hall |
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13:15 |
Poster Viewing |
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Disease (continued)
Chaired by John Draper, Aberystwyth University |
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14:00 |
Quality Assurance and Metabolite Identification in Metabolic Profiling: Considerations and Mass Spectrometric Applications in Pre-Eclampsia Studies
Warwick Dunn, Experimental Officer (Metabolomics and Mass Spectrometry), University of Manchester
Quality assurance and chemical identification of metabolites are two requirements to obtain valid, robust and applicable biological data in metabolomics. Approaches employed at The University of Manchester will be described with relevant examples taken from investigations into the pre-eclampsia syndrome. |
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Functional Genomics and Data Processing
Chaired by John Draper, Aberystwyth University |
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14:30 |
Towards Automated Signal Annotation in Mass Spectrometry Profile/Fingerprint Data
John Draper, Professor, Aberystwyth University
A database tool for semi-automated annotation of m/z signals in LC-MS data is described. The presentation will show how annotation predictions can be improved by converting external database structural information to a common format and by searching for all likely ionisation products. |
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15:00 |
Poster Award |
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15:10 |
Coffee Break and Networking in the Exhibition Hall |
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15:50 |
ANOVA-PLS: Improving the Analysis for Designed Studies
Uwe Thissen, Scientist Multivariate Statistics, TNO Quality of Life
Analyzing data from complex studies requires a comprehensive approach that combines many disciplines. An important aspect therein is combining statistics with prior design information. |
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16:20 |
Comparative GC-TOF-MS Time-Course Metabolite Profiles of Murine TNF- alpha Secreting and Non-Secreting Streptomyces Lividans TK24 Strains
Yankuba Kassama, Postdoctoral Research Associate, University of Manchester
Metabolic profiles of Streptomyces lividans TK24 show time-effect changes and differences between murine TNF-α expressing and non-producing strains in 47 metabolites. No significant differences were detected between the non-producing wildtype and empty pIJ486 strains. |
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16:50 |
Close of Conference | |