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Agenda

Day One - 15 May

07:30	Registration
08:30	DNA Microarrays: Applications and Experimental Design Chaired by Colin Campbell, EastChem Research Fellow, University of Edinburgh
08:35	How to Obtain Microarray Data You Can Believe Nigel Saunders, Reader in Microbiology, Sir William Dunn School of Pathology, University of Oxford Can you trust microarray data? Perhaps not – but it is possible! Strategies and methods needed to obtain reliable quantitative data - based on labelling techniques, slide manufacture, image extraction, channel correction, and exclusion of ‘non-data’ will be presented and discussed.
09:05	Non-Enzymatic Labelling of miRNA Using the Universal Linkage System (ULS™) Erik Jan Klok, Product Development Manager Microarrays, KREATECH Biotechnology B.V. ULS™ labels miRNA molecules directly in their natural form, without enzymes or linkers, and independent of their small size. The labelling reaction can be down-scaled to any low amount of miRNA (no limitations) and is compatible with many miRNA microarray platforms.
09:35	Formation and Switching of a Molecular Machine as a Label-Free, Specific DNA Sensor Colin Campbell, EastChem Research Fellow, University of Edinburgh The DNA nanoswitch is a new type of probe for unlabelled DNA and RNA. The switch mechanism allows for sensitive discrimination of single base mismatches and is read using a FRET output.
10:05	Coffee Break and Networking in Exhibition Hall
10:50	Next Generation Microarray Assays: Enzyme-On-Chip, Dynamic Detection and More… Peer Stähler, CSO, febit Biotech GENIOM’s application driven microfluidic microarrays enable high end assay formats and meet the challenges of fast moving fields such as microRNA research with their extraordinary adaptability. Microfluidics and highly automated processing support melting curve imaging and multiple use of microarrays.
11:20	Break to join the main auditorium for the Plenary Session
11:30	Plenary Lecture Chaired by Andreas Manz, Head Analytical Chemistry, Institute of Spectrochemistry and Applied Spectroscopy (ISAS)
	Genomics: From Medicine to the Environment Craig Venter, Founder and President, J. Craig Venter Institute and the J. Craig Venter Science Foundation
12:30	Lunch and Networking in Exhibition Hall
13:30	Poster Viewing
14:10	DNA Microarrays: Toxicogenomics Chaired by Steven Bodovitz, Principal, BioPerspectives
14:15	Toxicogenomic Investigation in Male Reproductive Toxicity David Rouquié, Genomic Activities Coordinator, Bayer CropScience Conventional toxicology studies measure the effects of chemical exposure on an organism following prolonged treatment and rely on macroscopic and microscopic analyses. The inclusion of Toxicogenomics facilitates understanding of toxicological mechanisms and may be used to predict adverse events.
14:45	Analysis of the Gene Expression Response to Sulphur Mustard Exposure Using the Rosetta Resolver System Tim Bonnert, Principal Scientist, Rosetta Biosoftware The presentation focuses on the analysis of a public data set (GEO GSE1888) examining the analysis of the gene expression changes occurring within 24 hours following exposure to the chemical warfare agent Sulphur Mustard, which causes severe, often terminal, damage to skin, eyes and pulmonary tissues some 12-24 hours following exposure.
15:15	Coffee Break and Networking in Exhibition Hall
16:00	DNA Microarrays: Diagnostics and Clinical Studies Chaired by Steven Bodovitz, Principal, BioPerspectives
16:05	Will DNA Microarrays Finally Make it to the Clinic? Bertrand Jordan, Coordinator, Marseille Nice Genopole Contrary to high expectations, DNA arrays are penetrating only slowly into diagnostics. The reasons for this are diverse and have much to do with the difference in environment and objectives between the research and diagnostic laboratory.
16:35	Pathway Analysis of Differentially Expressed Genes in Patients with Acute Aortic Dissection Salah Mohamed, Laboratory and Group Leader of Cardiac Surgery, University SH-Lübeck Whether dissected aortic tissues present significant similarities in their gene expression pattern was studied. This was also the opportunity to look for genes involved in the inflammatory processes.
17:05	Global microRNA Profiling of the NCI-60 Cancer Cell Lines Using Novel LNA Microarrays and Bead-Based Multiplex Assays Thomas Litman, Head of Biomarkers, Exiqon / University of Copenhagen A novel LNA based microarray platform for quantification of miRNAs has enabled generation of global miRNA expression profiles for the NCI-60 panel of cell lines and uncovered molecular miRNA signatures that may predict the drug sensitivity of tumours.
17:35	Drinks Reception compliments of Lab-on-a-Chip.com

Day Two - 16 May

08:30	DNA Microarrays: Polymorphisms Chaired by Herbert Auer, Manager of the Functional Genomics Core, Institute for Research in Biomedicine
08:35	Low Input Array Comparative Genomic Hybridization Steven Roman, Senior Scientist, Invitrogen Corporation The BioPrime® Total Genomic DNA Labelling System with novel nucleotide-dyes in a master mix format, which reduces pipetting and increases robustness, will be described. Data from BAC CGH arrays starting with as little as 50ng of genomic DNA will be presented.
09:05	Inter- and Intraplatform Reproducibility of Copy Number (CNV) Measurements Herbert Auer, Manager of the Functional Genomics Core, Institute for Research in Biomedicine DNA copy number variations (CNVs) are the newly discovered contributors to human genetic variation. The Microarray Research Group study presented here is the first broad evaluation of microarray platforms for CNV measurement.
09:35	Study of aCGH and SNP-VCN in Patients with Acute Lymphocytic Leukemia Rocio Ortiz-Lopez, Professor and Principal Investigator, School of Medicine, Universidad Autónoma de Nuevo León Ten ALL patients (T-ALL and B-ALL) were analyzed by aCGH to assess gain or loss cryptic regions. The mainly loosed region was 9p and the most frequently amplified region was 22q. B-ALL patients presented more consistent data than T-ALL patients.
10:05	Protein Microarrays: Interaction Chaired by Reinhard Hiller, Director, Centre for Proteomic & Genomic Research, University of Cape Town
10:10	Double-Detection Technology for Bioaffinity Experiments Simone König, Lecturer, Müenster University A strategy for combining the complementary techniques surface plasmon resonance biomolecular interaction analysis and matrix-assisted laser desorption/ionization mass spectrometry is reported. A special flow cell was constructed and removable pins carry the affinity-surface-bound analyte to the mass spectrometer. Functionalized pins can be assembled in target arrays.
10:40	Coffee Break and Networking in Exhibition Hall
11:20	Allergen Microarrays: From a Versatile Research Tool Towards a Promising Novel Diagnostic Application Reinhard Hiller, Director, Centre for Proteomic & Genomic Research, University of Cape Town The opportunities and challenges of using these allergen microarrays as novel molecular diagnostic applications will be discussed and issues of how to translate innovative protein microarrays into the clinical practice will be addressed.
11:50	Infrared-Based Protein Detection Arrays (IPAQ) for Quantitative Proteomics Ulrike Korf, Scientist, German Cancer Research Centre The reverse phase protein microarray technology was advanced towards improved sensitivity permitting the quantification of individual proteins derived from as little as 20.000 cells. Thus, a quantitative analysis of RNA silencing experiments as well as systems biology studies are feasible.
12:20	Peptide Microarray Approaches for Proteases and Kinases – From Substrate Specificity to Cleavage Kinetics Juan Diaz-Mochon, Post-Doctoral Fellow, University of Edinburgh The use of a microarray platform for enzymatic assays which allowed 10,000 peptide substrates to be screened with different proteases and kinases under identical conditions as well as the use of peptide arrays to study enzyme kinetics using real-time monitoring will be discussed.
12:50	Lunch and Networking in Exhibition Hall
13:30	Poster Viewing
14:10	Protein Microarrays: Antibodies Chaired by Carl Borrebaeck, Director of CREATE Health - a Strategic Centre for Translational Cancer Research, Lund University
14:15	Recombinant Antibody Microarrays - Focussed and Global Approaches for High-Throughput Proteomics Carl Borrebaeck, Director of CREATE Health - a Strategic Centre for Translational Cancer Research, Lund University Recombinant antibody fragments have been engineered to fulfil the requirements for designing an antibody microarray platform. Design of both focused and global array approaches, as well as applications of a multiplexed microarray platform, for analyzing complex proteome in health vs. cancer, will be presented.
14:45	Quantum Leap Improvements in Sensitivity, Precision and Dilutional Recovery in Multiplex Immunoassays Robert Negm, Vice President, GenTel BioSciences, Inc. GenTel BioSciences routinely measures multiple protein concentrations in serum samples obtained from clinical trials. We have significantly improved our process of development and validation of quantitative multiplex immunoassays.
15:15	Coffee Break and Poster Ceremony
15:45	Making it Small: Protein Microarrays for Tumour Analysis Markus Templin, Head of Microarray Technology, NMI Natural and Medical Sciences Institute Reverse Phase Proteinarrays are miniaturised systems capable of detecting high numbers of marker proteins from limiting amount of sample. Showing how pathological changes in cellular signalling networks can be detected and differences in phosphorylation of signalling proteins are used to define classes of tumours.
16:15	Can Protein Microarrays do the Trick in a Diagnostic Setting? Chamindie Punyadeera, Senior Scientist, Philips Research Laboratories The development of a rapid, multiplex protein detection platform for point-of-care-diagnosis will be presented. The data will be shown on the sensitivity and specificity of the flow-through immunoassay.
16:45	Close of Conference