|
Day 1 - |
Wednesday 17th September 2008 |
|
08:55 |
qPCR Diagnostics
Session Chair: |
|
09:00 |
RT-qPCR: Conjuring trick or gold standard ?
Stephen Bustin, Professor, University of London
How valid is the description of RT-qPCR as the gold standard for the quantification of nucleic acids? There is an urgent need to acknowledge and address the problems associated with this technology. This presentations will outline the main issues and propose appropriate solutions. |
|
10:00 |
Real-Time PCR Expression Profiling
Mikael Kubista, Doctor, TATAA Biocentre |
|
10:30 |
Coffee & Networking in Main Exhibition Hall |
|
11:30 |
RNAi Technology Trend Mapping Highlights
Gary Oosta, Chief Scientific Officer, Oval Ideas Inc
Mapping scientific and patent publications can reveal trends that can guide R&D decisions. Our map of the RNAi space is constructed by segmenting the collection of both scientific and patent documents into groups that describe research focus areas (activities), technology-stack layers (developments) and business or market risks and opportunities (interests). Visualizations across the segments reveal comparative trends and market insights. Analysis of trends of reveals opportunities for collaboration, research, licensing, or acquisition. A top-level summary dashboard provides guidance for R&D management decisions. |
|
12:00 |
Gene Expression Profiling of Peripheral Blood of Patients with SCA1 and SCA3 Identifies Potential Disease Progression Markers
Michael Walter, Staff Scientist, University of Tübingen |
|
12:30 |
Lunch and Poster Presentations |
|
14:25 |
Assay QC
Session Chair: |
|
14:30 |
Inhibition of the PCR Reaction
Jim Huggett, Professor, University College London
PCR inhibition can be highly reaction specific. At the extreme it is possible for one PCR reaction to be unaffected by potential inhibitors while another is totally inhibited. |
|
15:00 |
Selection of Optimal Reference Genes for Normalization
Inna Chervoneva, Assistant Professor, Thomas Jefferson University |
|
15:30 |
Coffee & Networking in Main Exhibition Hall |
|
16:30 |
Gene Expression Profiles of Antioxidative Enzymes and Related miRNAs as a Tool to Identify Metal-Specific Effects in A. Thaliana
Karen Smeets, Assistant, Hasslet University |
|
17:00 |
Method for Extraction, Reverse Transcription and PCR Applicable to Single Cell Analysis
Linda Strömbom, Scientist, TATAA Biocenter
In this talk we present a novel streamlined approach for real-time PCR based RNA analysis of few cell samples of lower complexity. The procedure eliminates material losses and all target RNA molecules are made available for reverse transcription. |
|
17:30 |
A New Normalisation Method for Murine Gene Eexpression Analysis
Nina Witt, PhD Student, University College London
We are using expressed short interspersed nuclear elements (SINE) for normalising RT-qPCR data and compare them with multiple reference genes. |
|
18:00 |
Drinks Reception - For Sponsorship please contact paul.raggett@selectbiosciences.com |
|
Day 2 - |
Thursday 18th September 2008 |
|
08:30 |
Title to be Confirmed
Micro Castoldi, Professor, University of Heidleberg |
|
09:00 |
Impact of q-RT-PCR Aalytical Methods on a Multi-Center Biomarker Trial in Colorectal Cancer
Terry Hyslop, Associate Professor, Thomas Jefferson University |
|
09:30 |
The Evolution of Relative RNA Quantification
Michael Pfaffl, Principal Scientist in Molecular Physiology, Technical University Munich
The focus of the talk is to describe the improvements in relative RNA quantification achieved during the last decade of quantitative RT-PCR. Accuracy of the relative quantification procedure can be increased, by implementing the PCR efficiency correction, multiple reference genes normalization, proper error propagation and valid statistical testing. |
|
10:00 |
Potential Dagnostic Application for Copy Number Detection Using Quantitative PCR with TaqMan Assays
Kelly Li, Senior Staff Scientist, Applied Biosystems |
|
10:30 |
Coffee & Networking in Main Exhibition Hall |
|
11:30 |
Detection of the JAK2 V617F Mutation in Myeloproliferative Disorders Using Novel Plexor LNA Primers
Curtis Hughesman, PhD Student, University of British Columbia
The design and application of unique Plexor LNA primers to detect the Janus Kinase 2 (JAK2) V617F mutation in clinical samples is presented. This newly developed real-time PCR assay has a significantly improved detection limit over probe based assays.
|
|
12:00 |
Applications of qPCR in DNA Methylation Analysis
James Flanagan, Research Fellow, University College London
Current techniques for DNA methylation analysis rely heavily on qPCR. This presentation will discuss a gene specific methylation protocol called Methylight and a genome-wide approach called methlyated DNA immunoprecipitation (MeDIP). |
|
12:30 |
Lunch and Poster Presentations |
|
14:00 |
ZNAs: New High-Affinity Synthetic Oligonucleotides as Powerful Tools for PCR
Nathalie Lenne, Project Leader, Polypus Transfection |
|
14:30 |
Targeted Genome Sequencing
George Weinstock, Professor, Baylor College of Medicine
Next generation sequencing makes genome-wide projects now high-throughput and cost-effective. Many of these projects seek to target gene sets rather than the entire genome landscape. Traditional methods of PCR-based resequencing are being challenged by a new generation of "capture" methods where targeted regions are purified and sequenced on nexgen platforms. The current state and promise of some of these will be presented. |
|
15:00 |
High-throughput Primer Design and in Silico Assay Validation
Jo Vandesompele, Professor, Ghent University Hospital
RTPrimerDB is a one-stop portal for experimentally validated qPCR assays, in silico validation of custom assays and now also for high-throughput primer design. The design pipeline is applied to a new class of non-coding RNA molecules. |
|
15:30 |
Coffee & Networking in Main Exhibition Hall |
|
16:00 |
Histone Modifications at RARE-driven Target Genes by all-/Trans/Retinoic Acid (ATRA) and TNFa
Marina Tshuikina, PhD Student, Uppsala University |
|
16:30 |
New Aspects of Gene Expression Analysis on the LightCycler® Platform Superior Solutions for Flexible and Rapid High-throughput Real-time PCR Gene Expression Studies
Ralph Mauritz, Director R&D, Roche Diagnostics |
|
17:00 |
Close of Conference |