Introduction to Microarrays
This course will be held prior to the Advances in Microarray Technology conference.
Who Should Attend?
The course will be suitable for scientists, technicians and engineers that plan to start working with microarray. It is helpful to be familiar with genes, RNA and proteins to follow the course.
Learning Objectives
1. Master the basics of microarray including surface chemistries, fabrication, hybridisation and detection technologies
2. Understanding DNA microarray probe design for SNP detection and bacterial diagnostics
3. Fundamentals of gene expression analysis using DNA microarrays
4. Protein microarrays, fabrication and applications
Topics and Course Organisation
Fabrication and using microarrays
1. Immobilisation of DNA to different surfaces
2. Fabrication of microarrays
a. Contact printing
b. Non-contact printing
c. In situ synthesis of DNA
3. Hybridisation
4. Detection
a. Fluorescence
b. Light scattering of gold/silver particles
c. Colorimetric
Demonstration of the effects of stringency in SNP genotyping by allele specific genotyping
1. Possible application of DNA microarrays: linkage studies and diagnostics
2. Designing a microarray for bacterial diagnostics
3. Basics of allele specific hybridisation
4. Designing a DNA microarrays for SNP/small mutation detection as exemplified by the globin gene
5. Minisequencing/primer extension on arrays
Genexpression studies using DNA microarrays
1. Examples of applications
2. Platforms
a. Home made arrays/cDNA arrays
b. Affymetrix
c. Agilent
d. Illumina
3. Production of target
a. Priming methods
b. Reverse transcription
c. T7 amplification
4. Hybridisation formats; co-hybridisation and parallel hybridisation
5. Bioinformatics
6. MIAME and importance of repeating experiments
Protein microarrays
1. Comparison between DNA and protein microarrays
2. Fabrication issues
3. Applications
a. Immunoarrays
b. Protein arrays
i. Diagnostics (allergen microarrays)
ii. Proteome arrays
c. Small molecule arrays
Other types of arrays
1. Comparative genomic hybridisation
2. CHiP on chip assays
3. Transfecting cells on microarrays
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