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These courses were held in conjunction with Virtual Discovery and Advances in Protein Crystallography.
Fluorescence Assays in Drug Discovery
8.30am-4.30pm 21 January and 8.30am-12.30pm 22 January
What will I gain by attending ?
- Master the basic concepts of fluorescence at the level required to understand fluorometric assays and instrumentation.
- Become familiar with the application of fluorometric assays to the major classes of pharmacological targets.
- Understand the basis and applications of advanced methods such as fluorescence imaging, fluorescence polarization, time-resolved energy transfer, and fluorescence-fluctuation spectroscopy.
- Learn the important types of interferences in fluorometric assays.
- Discover where to find more advanced information, using the extensive list of references provided.
Enzyme & Binding Assays in Drug Discovery
1.30pm-5.00pm 22 January and 8.30am-4.30pm 23 January
What will I gain by attending ?
- Master the basic concepts underlying enzyme and binding assays
- Learn how applications in drug discovery sometimes dictate assay optimization and interpretation which differ from textbook recommendations
- Learn how best to extract the information you need from your assays
Topics for the Fluorescence course:
- Fluorescence fundamentals
- Labels and labeling chemistries
- Instrumentation
- Interferences and limitations
- Survey of principal fluorescence methods
- Biochemical and cellular applications
Topics for the Enzyme & Binding course:
- Enzyme kinetics
- Enzyme inhibition: Competitive, noncompetitive, uncompetitive, irreversible, and promiscuous
- Multiple-substrate enzymes
- Binding equilibria and kinetics
- Deviations from classical textbook behavior
- Optimizing enzyme and binding assays for primary vs. secondary screening
- Understanding mechanisms and extracting information from your data
- Discussion of commercial data-analysis and simulation software
- Case studies, including receptor binding, kinases, and proteases
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