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Provisional Agenda
(This agenda is subject to addition and change)

Day 1 - 19th June 2008

08:00  

Registration

08:55

Protein Chemistry and Crystallization
Chair: Naomi Chayen, Professor, Imperial College London

09:00

The 3.4 A Resolution X-ray Crystal Structure of a Plant Photosystem I Supercomplex
Alexey Amunts, PhD Student, Tel Aviv University
Photosystem-I is multi-subunit membrane supercomplex of protein and non-protein components that drives the photosynthesis process. The intact structure is described at 3.4Å resolution. The structural information provides a first glimpse at the fine architecture of Nature’s most efficient photochemical machine.

09:30

“Plain truth” of Crystallomic vs. “Heresy” of High Throughput Crystallization
Jan Sedzik, Associate Professor, Karolinska Institute
Comparison between technology of crystallomic, and high throughput crystallization.

10:00

Coffee & Networking in the Exhibition Hall

11:00 

New Laboratory Instrumentation and Techniques

 

New Insights into Biomolecule Crystallization Processes using Dynamic Light Scattering and Imaging in Multi-well Plates
Christian Betzel, Professor, University of Hamburg
Automated methods to crystallize macromolecules are widely used and can easily generate thousands of crystallization droplets. Nevertheless, evaluation of crystallization experiments to find optimal growth conditions remains a bottleneck.

11:30

Towards the Design of a 'Universal' Nucleant for Protein Crystallization
Naomi Chayen, Professor, Imperial College London
This talk will evaluate the use of nucleants for protein crystallization and examine the developments in the quest to develop nucleants for both the screening and optimisation phases of crystallisation.

12:00

Integrating Structural Bioinformatics and Proteomics Approaches to Discover New Genes, Protein Families, and Function in Apoptosis and Inflammation
Frederic Pio, Assistant Professor, Simon Fraser University
Identifying new genes and members of a protein family, and characterizing their structure and function is fundamental to obtaining a complete list of the components that constitute our genomes

12:30     

Lunch & Poster Viewing

TTP Labtech Workshop

14:30      

Advances in Diffraction Techniques and Crystallographic Computing

 

Fully Remote Access to SSRL Protein Crystallography Beam Lines
Clyde Smith, Staff Scientist, Stanford University
During the last user run (2007/2008), over 75% of the user groups have used the remote access tools available on the six protein crystallography beam lines at SSRL. This live remote access demonstration will showcase the features currently available to remote users.

15:00

Automation, Robotics and Remote Access at the SSRL Protein Crystallography Beam Lines
Aina Cohen, Staff Scientist, Stanford University
The crystal screening and data collection component of the macromolecular crystallography experiment lends itself perfectly to automation. The design and implementation of the automated technologies enabling fully remote access to the SSRL macromolecular crystallography will be described.

15:30        

Coffee & Networking in Main Exhibition Hall

16:30

Database for Structural data Using Semantic Web concepts to Support Structure-Based Drug Design for AIDS
T.N. Bhat, Project Leader, National Institute of Standards and Technology
This presentation will illustrate how Semantic Web concepts are used for novel annotation, data integration, storage, and query to manage and display structural data.

17:00    

Multi-Plane Rotational Imaging and Automated Crystal Harvesting
Thomas Friedlander, President, FM Pharmaceutical Products
This presentation will look at new ways to image crystals and harvest them in an automated or semi-automated method. With the advent of 50nl-100nl drops and smaller and smaller crystals being mounted and analyzed a system for assisting the researcher with preparing and visualizing these crystals will be examined.

17:30

Drinks Reception in Main Exhibition Hall - Sponsorship available, contact paul.raggett@selectbiosciences.com



Day 2 - 20th June 2008

08:55

Advances in Diffraction Techniques and Crystallographic Computing
Chair:
Sir Tom Blundell, Professor of Biochemistry and Chair, School of Biological Sciences, University of Cambridge

09:00         

High-Throughput Protein Structure Determination Using Computational Grids 
Ashley Buckle, Group Leader, Monash University
We have developed a simple web-based approach to testing tens to hundreds of molecular replacement (MR) search models using a modest lab-based grid. The second approach entails exhaustive MR using the entire PDB. All software is open source.

09:30

Crystal Structure of CapB, a Tyrosine Kinase from the Pathogen Staphylococcus Sureus
Sylvie Nessler, Professor, LEBS, CNRS Gif sur Yvette
This work presents the structural analysis of a bacterial tyrosine kinase  implicated in the pathogenicity of the human pathogen Staphylococcus aureus. It allowed us to explain the catalytic and regulation mechanisms of this interesting drug design target.

10:00

Coffee & Networking in Main Exhiition Hall

11:00

High-Throughput Protein Crystallography and Drug Discovery
Sir Tom Blundell, Professor of Biochemistry and Chair, School of Biological Sciences, University of Cambridge
The knowledge of the three-dimensional structures of protein targets that is now emerging from structural proteomics and targeted structural biology programmes has the potential to increase our understanding of human genetic variation, as well as to accelerate drug discovery. 

11:30

A Repository for Protein Structural Information
Howard Feldman, Senior Research Scientist, Chemical Computing Group
PSILO is a database system that provides an easily accessible, consolidated repository for protein and protein-ligand structural information. It offers means to register both experimental and computational macromolecular structural data, and access it through a web interface.

12:00

Lunch & Poster Presentations

14:00

Probing the Substrate Specificity and Mechanism of EstB Esterase from Lactobacillus Rhamnosus
Gillian Norris, Senior Lecturer, Massey University
Structures of EstB, an esterase from the dairy lactic acid bacterium Lactobacillus rhamnosus have been solved in the presence of a number of substrate analogue inhibitors.  Comparisons of the rates of inhibition and enzyme-inhibitor structures have provided an insight into the substrate specificities and mechanism of EstB.

14:30

A Rapid Method for Determining Shape and Cross Sections of Macromolecular Complexes
Iain Campuzano, Application Research Scientist, Waters Corp
Over the past 10 years interest in macromolecule protein mass spectral analysis has increased due to the ability of the current mass spectrometers and electrospray sources to preserve the non-covalent protein/protein subunit interactions, allowing one to analyse proteins in their native conformation and stoichiometry. By coupling mass spectrometry with ion mobility, not only can the intact quaternary mass of biological macromolecules be accurately mass measured, but also the shape (collisional cross section) of the biological macromolecule inferred. Differences in shape and structure upon binding of a substrate can also be rapidly measured by ion mobility (IMS).

15:00

Coffee & Networking in Main Exhibition Hall 

16:00

The PX Scanner: In-situ X-Ray Inspection of Protein Crystals in multi-Well Plates
Marcus Winter, Manager, Oxford Difraction
A quantitative study on the effect of radiation damage during the acquisition of X-ray data from protein crystals at room temperature will be presented.

16:30

Close of Conference