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Agenda
Day 1 - 4 September
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09:30 |
Quantitative Proteomics
Chaired by Dolores J. Cahill, Professor of Translational Science, University College Dublin |
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09:35 |
Quantitative Proteomics and Systems Biology
Ruedi Aebersold, Professor, ETH Zurich
Attempts to comprehensively analyze the proteome of eukaryotic cells, the challenges such projects face and outline strategies to overcome them will be discussed. |
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10:05 |
Coffee Break and Networking in Exhibition Hall |
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10:50 |
Biological Reference Materials for Discovery and Validation of Biomarkers in Tissues and Body Fluids
Peter Schulz-Knappe, Director, Proteome Sciences
Lack of precision and reproducibility hamper proteomics analysis. Spiking of biological reference materials labelled with Tandem Mass Tags™ allows for relative quantification of the entire sample proteome with highly improved precision and CV. |
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11:20 |
Strategies for Proteome Simplification and Quantification
Robert Beynon, Head, Proteomics and Functional Genomics Group, University of Liverpool
That proteomics requires novel thinking and novel strategies to become quantitative and global. |
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11:50 |
Lunch and Networking in Exhibition Hall |
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13:00 |
Poster Viewing |
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13:45 |
Post-Translational Modifications: Glycomics
Chaired by Robert Beynon, Head, Proteomics and Functional Genomics Group, University of Liverpool |
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13:50 |
Peptides and Glycans for the Detection of Hepatocellular Carcinoma
Radoslav Goldman, Assistant Professor, Georgetown University
Analyses of peptides and glycans represent newly tested strategies for discovery of cancer biomarkers. The application of these methods to the detection of hepatocellular carcinoma identified a set of candidate biomarkers that classify HCC with greater than 90 % prediction accuracy. |
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14:20 |
Chemical Probes for the Detection of Carbohydrate Binding Proteins
Roland Pieters, Associate Professor, Utrecht University
Protein-carbohydrate interactions determine important biological processes, many of which are linked to disease. Chemical probes were prepared that enable the detection, ultimately for diagnostic purposes, of certain carbohydrate binding proteins from protein mixtures. |
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14:50 |
Tools for Glycomics: Methods for Relative Quantitation of Glycans and Biomarker Discovery Using Lectin Glycoproteomics
Michael Pierce, Professor, University of Georgia
Glycosylation is the most complex post-translational modification of proteins, influencing and regulating diverse physiologic processes. Analysis of glycan structures is an important component of the emerging field of glycoproteomics, and the use of mass spectrometry has enabled the investigation of the glycan repertoire of organisms. |
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15:20 |
Coffee Break and Networking in Exhibition Hall |
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16:05 |
Protein Expression
Chaired by Roland Pieters, Associate Professor, Utrecht University |
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16:10 |
Proteomic Scale Generation and Validation of Recombinant Antibodies
John McCafferty, Senior Project Leader/Principal Investigator, Wellcome Trust Sanger Institute
A consistent source of monoclonal antibodies to the wealth of newly discovered genes in the genome would be of huge value to the research community. We demonstrate the feasibility of this by selecting and characterising 7000 unique recombinant antibodies to 300 antigens. |
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16:40 |
High Throughput Generation of Baculovirus Expression Vectors
Robert Possee, Group Leader, CEH Oxford
An automated high throughput system for the generation of recombinant baculoviruses has been produced that permits parallel construction of expression vectors in insect cells, without the need for plaque purification. The process involved is essentially a one-step transfection. |
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17:10 |
Building the Cell Cycle Interactome by Protein Complex Purifications from Plant Cell Suspension Cultures
Geert De Jaeger, Professor, VIB Dept. Plant Systems Biology, Ghent University
A high throughput TAP based technology platform was raised to isolate protein complexes from plant cell suspension cultures. It is currently applied to gradually build a cell cycle interactome for Arabidopsis thaliana. Proof of concept for complex isolation will be shown for a set of cell cycle related proteins. |
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17:40 |
Drinks Reception Compliments of Dionex |
Day 2 - 5 September
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09:00 |
Disease Proteomics
Chaired by John McCafferty, Senior Project Leader/Principal Investigator, Wellcome Trust Sanger Institute |
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09:05 |
Quantitative LC-MS Serum Analysis for Gaucher Disease
Hans Aerts, Professor, Academic Medical Centre, University of Amsterdam
A label-free LC-MS method, including novel analysis concepts, has been described for absolute and relative quantification of Gaucher disease related biomarkers and indicators in serum. The LC-MS activity levels were found to be in good agreement with biochemical assay levels. |
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09:35 |
Protein Extraction from Formalin-Fixed Clinical Tissues for Nanoscale Biomarker Analysis
Karl-Friedrich Becker, Professor, Technical University of Munich - Institute of Pathology
A novel technology for isolation of intact, soluble and immunoreactive proteins from formalin-fixed tissues has been developed. Applications of this method for measuring abundances of protein markers and profiling of deregulated signalling pathways in various diseases will be presented. |
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10:05 |
Biomarker Discovery and Validation in Biomedicine/Clinical Proteomics
Steve Pennington, Professor of Proteomics, Conway Institute for Biomolecular and Biomedical Research, University College Dublin
After a brief introduction to biomarkers, from discovery to clinical utility, the application of different proteomics strategies in recent and on-going biomarker discovery projects in pancreatic and prostate cancer and predictive toxicology will be presented. |
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10:35 |
Coffee Break and Networking in Exhibition Hall |
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11:20 |
Proteomics Analysis of Alveolar Macrophages in Sarcoidosis Patients Suggests an Increased Apoptosis Rate and a Dysregulation of Iron Metabolism
Elisabetta Fortunati, Senior Scientist, University Medical Centre Utrecht
Proteomic technology is a powerful tool for the identification of novel prognostic, diagnostic and therapeutic markers. Its application in the study of Sarcoidosis yield novel information about the pathophysiology of this incurable lung disease. |
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11:50 |
Towards a Database of Breast Cancer Proteomics: Proteome Profiling of Human Breast Cancer Showed Unique Pattern of Protein Expression and Phosphorylation
Serhiy Souchelnytskyi, Associate Professor, Head of Laboratory, Karolinska University Hospital
Proteomics proved to improve cancer diagnostics and treatment. Cancer-related changes in proteome of human breast epithelial cells are searched for. These proteins are analyzed by systems biology tools and can be used as markers and drug targets. |
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12:20 |
Lunch and Networking in Exhibition Hall |
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13:30 |
Poster Viewing |
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14:15 |
Proteomics Technologies
Chaired by Steve Pennington, Professor of Proteomics, Conway Institute for Biomolecular and Biomedical Research, University College Dublin |
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14:20 |
Interaction Screening On Protein Microarrays
Dolores J. Cahill, Professor of Translational Science, University College Dublin
Technology improvements towards measuring interactions on protein microarrays will be presented. |
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14:50 |
Poster Award and Coffee Break |
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15:20 |
Integration and Cross-Analysis of Data - How it Can Aid Understanding of Cancer
Marketa Zvelebil, Group Leader: Bioinformatics & Systems Biology, Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine
pSTIING is a new publicly accessible web-based application and knowledgebase of molecular associations. It also supports the direct cross-correlation of experimental results with interaction information in the knowledgebase, which currently analyses and clusters gene expression, proteomic and phenotypic datasets. |
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15:50 |
The MAPPIT Toolbox: New Strategies for the Analysis of Molecular Interactions in Mammalian Cells
Jan Tavernier, Professor, VIB Dept. Medical Protein Research, Ghent University
MAPPIT (Mammalian Protein-Protein Interaction Trap) is a cytokine receptor-based two-hybrid method that operates in intact mammalian cells. Applications and variations of the MAPPIT concept will be discussed. |
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16:20 |
Close of Conference | |