Free Lunchtime Workshop
17 September 2009
Genome-Wide siRNAi Screening Using the Acumen eX3 – A Customer Perspective from Cancer Research UK
Dr Sarah Payne, Product Manager, TTP LabTech Ltd and Dr Michael Howell, Head of the High Throughput Screening Laboratory, Cancer Research UK
TTP LabTech’s Acumen eX3 fluorescent microplate cytometer enables the quantification of high content assays at high throughput and has been successfully used in many genome-wide RNAi studies. In this tutorial, the features of Acumen eX3 that have led to its global adoption by researchers in this area will be introduced, followed by a presentation by Dr Michael Howell, from the high throughout screening facility at Cancer Research UK’s (CRUK) London Research Institute, outlining his involvement in two on-going functional genome wide siRNA projects.
Screen one investigates the molecular mechanisms of PKC-induced activation of ADAM proteases by measuring ectodomain shedding of a HB-EGF fusion protein. The second functional screen aims to determine genomic instability, survival and drug resistance pathways in human cancer by measuring changes in ploidy, cell survival and apoptosis with a view to establishing more effective combinatorial therapy regimes.
