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Agenda
Day One - April 24
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8:00am |
Registration |
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9:00am |
RNAi - Basic Biology and Tool Development
Session Chair: Dmitry Samarsky, Director, Technology Development, Dharmacon/ThermoFisher |
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9:05am |
Gene Silencing with RNAse P and External Guide Sequences (EGS) for Therapy of Atopic and Respiratory Diseases
Lucy Ghoda, Assistant Professor, the Webb-Waring Institute
Small RNA molecules termed external guide sequences (EGS) resemble the structure of tRNA when bound to mRNA. EGS can target mRNA for degradation by the RNA enzyme, RNase P and could be used for gene silencing in humans. |
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9:35am |
Keynote Address - Title to be Confirmed
Nicholaus Rajewsky, Full Professor and Head, Division of Systems Biology, Max Delbruck Center for Molecular Medicine |
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10:20am |
Coffee & networking in exhibition hall |
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Use of the Nucleofector 96-well Shuttle System for successful RNAi experiments in difficult-to-transfect cell types
Ludger Altrogge, Senior Scientist, Amaxa |
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A Low-copy Sense Stranded Promoter RNA is Required for siRNA Mediated Transcriptional Gene Silencing in Human Cells
Kevin Morris, Assistant Professor, Department of Molecular and Experimental Medicine, The Scripps Research Institute |
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12:05pm |
Use of Dicer-substrate siRNAs in vitro and in vivo
Mark Behlke, VP Molecular Genetics, Integrated DNA Technologies
Dicer-substrate RNAs (DsiRNAs) can be 10-fold or more potent than the corresponding 21mer species. Dicer-substrate duplexes function well in vivo and the utility of these compounds in several mammalian model systems will be presented. |
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12:35pm |
Lunch & poster viewing |
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2:05pm |
microRNA - Detection, Profiling and Function Analysis
Session Chair: Mark Behlke, VP Molecular Genetics, Integrated DNA Technologies |
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Genome-wide microRNA Profiling of Frozen and Formalin-Fixed Paraffin-Embedded Tissues
Rajiv Raja, Director, Molecular Biology, Molecular Devices |
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Keynote Address - Genome-wide microRNA Profiling of Frozen and Formalin-fixed Paraffin-embedded Tissues
Reuven Agami, Associated Professor, The Netherlands Cancer Institute |
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3:25pm |
Coffee & networking in exhibition hall |
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Efficient and specific quantification of mammalian microRNAs by a novel real-time PCR approach
Eric Lader, Director, QIAGEN
This technology offers researchers a very sensitive, specific, and easy to carry out approach for accurate expression profiling of miRNAs using a small amount of total RNA containing microRNAs. |
| 4:40pm |
microRNA-378 Promotes Cell Survival and Tumor Growth by Targeting Sufu and Fus-1 Expression
Burton Yang, Senior Scientist/Associate Professor, Sunnybrook Health Sciences Centre
An unique system for microRNA expression has been developed, which allows effective expression of a microRNA and generation of stable cell lines expressing the microRNA for functional studies. |
| 5:10pm |
High Throughput microRNA Expression Profiling Using a Bead Based Multiplex System
Keld Sorensen, Director of Research and Development, Luminex |
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5:40pm |
Drinks reception in exhibition hall |
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Day Two - April 25
| 9:00am |
RNAi in Gene Function Analysis and Target Identification
Session Chair: Tod Woolf, President, RXi Pharmaceuticals |
| 9:05am |
High Throughput RNAi Screening: Initial Steps Toward Creation of a Functional Map of the Human Genome
Dmitry Samarsky, Director - Technology Development, Dharmacon/ThermoFisher
Successful merging of RNAi into high throughput applications requires the identification of critical attributes that distort the outcome of gene silencing studies. To that end, this presentation will review results of a recent multi-laboratory high throughput RNAi phenotypic screen. The importance of assay design, reagent quality, positive and negative controls, and assay metrics will be discussed. Consideration of these attributes will minimize the complexities of hit stratification and facilitate the development of an accurate functional map of the human genome. |
| 9:35am |
Identification of Host Cell Factors Affecting Bacterial Infection
Nikolaus Machuy, Team Leader Screening Facility, Max Planck Institute for Infection Biology |
| 10:05am |
Coffee & networking in exhibition hall |
| 10:50am |
A Complete New Design for siRNA Screening
Quick Que, Head of Computational Pharmacogenomics, Translational Genomics Research Institute |
| 11:20am |
Integrating a Novel Imaging Technique with RNAi to Investigate the Heme Biosynthetic Pathway
Sarah Haigh, Senior Scientist, Molecular Cytomics |
| 11:50am |
Keynote Address - RNAi Mediated Inhibition of Gene Expression at the Transcriptional and Post-transcriptional Levels
John Rossi, Director, City of Hope National Medical Center |
| 12:35pm |
Lunch & poster viewing |
| 2:05pm |
Development of RNAi System to Study Signal Transduction in Primary Human B Cells
Joanne Kamens, Group Leader, Abbott |
| 2:35pm |
RNAi In Vivo Applications and Therapeutics
Session Chair: Joanne Kamens, Group Leader, Abbott |
| 2:40pm |
Delivery of Intact RNAi to Target Tissues In Vivo
Tod Woolf, President, RXi Pharmaceuticals |
| 3:10pm |
Coffee & networking in exhibition hall |
| 3:55pm |
Anti-inflammatory Activity of a Liposomal CD40 Antisense Oligo-Nucleotide in a Model of Rheumatoid Arthritis
Steffen Panzer, CEO/CSO, Novosom |
| 4:25pm |
Cancer Therapy by RNAi in the Tumor Vascular Endothelium After Intravenous Administration of siRNA-Lipoplexes
Ansgar Santel, Senior Scientist, Atugen
Liposomally formulated siRNA, but not naked siRNA can be used in vivo for RNAi in the vascular endothelium, when applied intravenously, opening the opportunity to develop a novel therapeutic modality for oncology and other diseases. |
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4:55pm |
Close of Conference | |