Gene Expression
This course will be held prior to the Advances in Microarray Technology Conference.
Topics and Course Organisation
Gene expression studies using DNA microarrays
1. Experimental design:
a. Single vs Dual channel considerations
b. Descriptive and ‘fishing’ studies
c. Systems biology / functional genomics studies
2. Platforms:
a. ‘Home made’ arrays
b. Affymetrix
c. On-slide synthesized oligoarrays (such as Agilent and Nimblegen)
3. Production and labeling of the target:
a. Quality control issues
b. Labeling methods
c. Amplification
4. Hybridization conditions influencing performance, sensitivity, and specificity.
5. Optimal image acquisition and extraction.
6. Data analysis.
7. Data management, publication / validation issues, and MIAME compliance