Abstract

Lab-on-Chip Devices For Analysis of Extracellular Vesicles and Their Contents From Cerebral Organoid Tissue

Holger Schmidt, Narinder Kapany Professor of Electrical Engineering, University of California-Santa Cruz

Cultured organoids have emerged as important test beds for studying cell development and disease. Sub-micron sized extracellular vesicles (EVs), including exosomes, secreted by these organoids provide an ideal opportunity for assessing and continuously monitoring organoid status on the molecular level. I will describe the development of integrated lab-on-chip devices for real-time analysis of EVs produced in a custom 3D cerebral organoid tissue culture platform. This includes the detection of individual exosomes as well as their molecular biomarker cargo using single particle fluorescence and trapping-assisted nanopore sensing. I will also discuss the direct incorporation of this electro-optofluidic lab-on-chip within the tissue culture platform.