Shopping Cart (0)
My Account

Shopping Cart
SELECTBIO Conferences Single Cell Analysis Summit 2018

Paul Bohn's Biography



Paul Bohn, Arthur J. Schmitt Professor of Chemical and Biomolecular Engineering and Professor of Chemistry and Biochemistry, University of Notre Dame

Paul W. Bohn received the B.S. in Chemistry from the University of Notre Dame in 1977 and his Ph.D. in Chemistry from the University of Wisconsin-Madison in 1981. After two years at Bell Laboratories, Murray Hill, NJ, he joined the faculty at the University of Illinois at Urbana-Champaign (UIUC). In 2006, he left UIUC for the University of Notre Dame where he is currently the Arthur J. Schmitt Professor of Chemical and Biomolecular Engineering, Professor of Chemistry and Biochemistry, and Director of the Advanced Diagnostics & Therapeutics Initiative. He served as Editor for the Americas for the RSC journal Analyst 2007-09 and as Chair of the Editorial Board for 2010-14. Prof. Bohn has served on numerous editorial and scientific boards and is currently co-editor of Annual Review of Analytical Chemistry. His research interests include: (a) integrated nanofluidic and microfluidic chemical measurement strategies for personal monitoring, (b) chemical and biochemical sensing in mass-limited samples, (c) biochemical imaging, and (d) molecular approaches to nanotechnology, areas in which he has over 260 publications and 6 patents.

Paul Bohn Image

Zero-Mode Waveguide Spectroelectrochemistry of Single Oxidoreductase Enzyme Molecules

Wednesday, 3 October 2018 at 09:30

Add to Calendar ▼2018-10-03 09:30:002018-10-03 10:30:00Europe/LondonZero-Mode Waveguide Spectroelectrochemistry of Single Oxidoreductase Enzyme MoleculesSingle Cell Analysis Summit 2018 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com

Single electron transfer events in both immobilized and freely diffusing redox-active enzymes can be imaged with facility using electrochemical zero-mode waveguide (E-ZMW) arrays. These bimodal nanoelectrochemical-nanophotonic nanopore arrays are composed of high density zeptoliter-volume recessed dual-ring electrode nanopores. Thus, they provide a link between single electron-transfer events and light emission in fluorigenic redox reactions, such as those involving flavin-containing enzymes, i.e. flavoenzymes. The bimodal optical-electrochemical functionality of the E-ZMW makes it possible to perform single molecule spectroelectrochemical measurements under conditions where the enzyme is potential controlled and optically-coupled, while the enzyme substrate is generated in situ electrochemically at a nearby second working electrode.   Thus, the E-ZMW makes it possible to study single enzyme turnover events in an environment where both confinement and molecular crowding may be controlled.  The ability to combine electrochemical and spectroscopic measurements at the single molecule level is a new tool for the characterization of reaction dynamics.


Add to Calendar ▼2018-10-02 00:00:002018-10-03 00:00:00Europe/LondonSingle Cell Analysis Summit 2018Single Cell Analysis Summit 2018 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com